拮抗水霉菌株的筛选及抑菌蛋白的分离纯化

Selection of Antagonistic Bacteria for Saprolegnia parasitica and Isolation of Its Antibiotic Protein

水霉病是一种广泛存在于水体中的真菌性病原菌,无寄主选择性,目前渔业生产上主要依赖化学药物防治,导致病原菌的抗药性增强.本研究通过平板对峙培养法,筛选到5株具有拮抗活性的菌株.通过硫酸铵盐析、葡聚糖凝胶和离子交换树脂等方式进行分离纯化,从Bacillus subtilis strain TCCC11201发酵液中分离得到一个对水霉病菌具有抑制活性的蛋白,经SDS-PAGE电泳检测,其相对分子质量为2×104.菌株TCCC 11201代谢产生的活性蛋白具有防治水霉病的潜在应用价值.

Saprolegnia parasitica is a pathogenic fungal of the fish, which is prevalence without host selectivity. Recently, it was prevented and treated mainly depend on the chemicals. Five bacterial from the strain bank of our laboratory were sieved out with the dural culture method. A kind of protein was separated out from the fermentation broth of the Bacillus subtilis strain TCCC 11201 which can inhibit the growth of water mold through sephadex gel, ion exchange resin and so on. After detected with SDS-PAGE protein electrophoresis, its molecular weight is about 2×10^4. This protein has potential appli- cation for the prevention and treatment of saprolegnia.