结直肠癌组织中GITRL mRNA表达水平的检测及其临床意义

Detection and significance of human GITRL mRNA in colorectal cancer tissues

目的:建立检测人糖皮质激素诱导的肿瘤坏死因子受体配体(human glucocorticoid induced tumor necrosis factor receptor ligand,hGITRL)mRNA的实时荧光PCR方法,探讨hGITRL表达与结直肠癌发生发展的关系。方法:构建含hGITRL基因片段的标准质粒,通过实时荧光PCR检测20例结直肠癌组织与对应癌旁组织中GITRLmRNA的表达水平。结果:建立的hGITRLRT-PCR基因定量检测方法准确可靠(标准曲线相关系数为0.99983);在20例结直肠癌组织细胞中,GITRL表达水平较之对应的癌旁组织有所升高(P<0.05),且与肿瘤组织分型和临床分期存在相关性。结论:成功建立了检测hGITRLmRNA的实时荧光定量PCR方法,GITRL在结直肠癌组织细胞中表达量升高,且与癌症的严重程度呈现一定的相关性。

Objective： To establish a method of real-time fluorescence PCR for human glucocorticoid induced tumor necrosis factor receptor ligand（hGITRL） mRNA and investigate the relationship between the expression of hGITRL gene and colorectal cancer occurrence.Methods： The standard plasmid of hGITRL gene was firstly constructed.The levels of expression of GITRL in colorectal cancer tissues（n=20） were detected by real time PCR.The correlation between GITRL and colorectal cancer occurrence were analysed.Results： The real-time PCR of GITRL was accurate and reliable.The expression of GITRL in colorectal cancer tissues increased compared with control（P0.05）.Conclusion： The real-time-PCR method for the detection of GITRL mRNA was establish successfully.The expression of GITRL in colorectal cancer tissues increased compared with control expression by real time PCR.Moreover,the correlation analysis with colorectal cancer occurrence and development suggested that it shares associativity with severity of colorectal cancer.