摘要
木聚糖酶在饲料、食品、纺织、造纸等行业应用广泛,是重要的工业用酶之一。利用丝状真菌—好食脉孢霉(N.sitopHila)为研究对象,以蛋白胨、豆渣、麸皮水和豆粕为底物进行液态发酵生产木聚糖酶,发酵后用滤纸真空抽滤后得粗酶液。用DNS法对脉孢霉半纤维素酶活力进行测定,对脉孢霉产木聚糖酶最优发酵培养基、接种量和培养温度进行研究,研究表明好食脉孢霉产半纤维素酶最适发酵培养基为3%麸皮水、0.5%蛋白胨、6%豆渣、pH自然,最适接种量为1%,最适发酵温度为28℃。
As one of the most important enzymes, xylanase has been widely applied in the fields of feed, food, textile and paper industries. The filiform fungus-neurospora sitophila was used as object of study, and xylanase was produced with using peptone, bean dregs, bran-water and bean pulp as substrates by liquid state fermentation. The raw enzyme liquid was obtained by vacuum filtration after fermentation. The activity of hemieellulase of neurospora sitophila was determined with DNS method. The optimal fermentation medium, inoculating dosage and culture temperature for producing xylanase by hemicellulase was studied. The results showed that the best fermentation medium contained 3%bran-water, 0.5%peptone and 6%bean dregs, and the optimum inoculating dosage was 1%, and the process should be carried out under the conditions of natural pH at 28℃.
出处
《黑龙江科学》
2011年第4期18-20,38,共4页
Heilongjiang Science
关键词
木聚糖酶
好食脉孢霉
液态发酵
Xylanase
neurospora sitophila
liquid state fermentation.
