摘要
目的:观察外源性骨髓间充质干细胞(Mesenchymal stem cells,MSCs)对庆大霉素(Gentamycin,GM)诱导的大鼠急性肾损伤是否具有治疗作用,并初探其机制。方法:建立腹腔注射庆大霉素致大鼠急性肾损伤模型实验分为正常对照组、模型组、MSCs治疗组(模型+MSCs)、生理盐水组(模型+生理盐水)。于不同处理后4d分别检测血尿素氮(BUN)和肌酐(Scr)水平,观察肾组织病理改变,免疫印迹及RT-PCR法检测肾组织肝细胞生长因子(Hepatocyte growth factor,HGF)水平。结果:模型组大鼠的BUN及Scr较正常对照组显著升高,且肾小管组织病理损伤严重;而MSCs治疗组大鼠的BUN及Scr水平较生理盐水组显著降低,肾小管组织病理损伤明显减轻。此外,促肾小管损伤修复的肝细胞生长因子(HGF)表达在MSCs治疗组显著高于生理盐水组。结论:MSCs输注可促进庆大霉素所致急性肾小管损伤的修复,改善肾功能,其作用机制可能是与上调肾组织中肝细胞细胞生长因子的表达有关。
Objective： To investigate whether the exogenous mesenchymal stem cells （MSCs） can promote the recovery of acute renal injury induced by gentamycin, and to explore possible mechanism. Methods： Acute renal injury rat model was established by intraperitoneal injection of gentamycin. SD rats were randomly divided into four groups which were normal control group, model group, MSCs injection group and saline group. The changes of rat renal function and pathology were observed four days after different treatment. The expression ofhepatocyte growth factor in kidney was examined by western blot and RT-PCR. Results： The levels of BUN and Scr in model group were much higher than those in the normal control group. While comparing with those in model group, the levels of BUN and Scr in the MSCs injection group were much lower. At the same time, MSCs infusion ameliorated the pathological changes significantly. Besides, the expression of growth factor HGF was obviously higher in MSCs-treated kidneys. Conclusions： MSCs can promote the recovery of acute renal tubular epithelial cells damage caused by gentamycin. The mechanism may partly depend on up-regulating the excretion ofhepatocyte growth factor in renal meroenvironment.
出处
《现代生物医学进展》
CAS
2011年第16期3025-3029,共5页
Progress in Modern Biomedicine
基金
徐州医学院药学院研究生科研创新计划资助项目(NO 2010YKYCX003)
