bFGF基因启动子区-921C＞G单核苷酸多态性与脊柱融合的相关性研究

The research of relationship between -921C>G single nucleotide polymorphisms(SNP) in bFGF gene promoter region and spinal fusion

目的研究山东半岛地区人群中,bFGF基因启动子区-921C>G单核苷酸多态性与脊柱融合的相关性。方法采用PCR及基因测序方法,检测225例脊柱融合患者外周血中bFGF基因C>G单核苷酸多态性情况,并根据术后不同时间随访X线的表现,分析其与脊柱术后植骨融合疗效之间的关系。结果病例组中均符合Hardy-Weinberg平衡检验(P>0.05),在病例组中,融合组和非融合组CC基因型与CG+GG基因型分布有统计学意义(P=0.026),融合速度CC基因型与CG+GG基因型分布在颈椎有统计学意义(P=0.025)。结论在山东半岛地区人群中,bFGF基因C>G单核苷酸多态性与植骨融合间存在显著性差异且与颈椎植骨融合速度之间存在相关性。

Objective To explore the relationship between -921C〉G single nucleotide polymorphisms（SNP） in bFGF gene promoter region and spinal fusion of the population in Shandong Peninsula. Methods Conducted with PCR and method of gene sequencing, the peripheral blood of 225 patients with spinal fusion was examined. The C〉G SNP of bFGF was checked. According to the postoperative manifestation detected through X ray in follow-up at different times, the relationship between C〉G SNP of bFGF and the postoperative efficacy of spinal fusion after bone graft was analyzed. Results All patients in case group were in line with Hardy-Weinberg equilibrium test （P〉0.05）. The case group was divided into two parts： fusion group and non-fusion group. In the case group, CC genotype and the distribution of CG＋GG genotype in both fusion group and non-fusion group had statistic significance （P=0.026）. With respect to the infusion speed, the distribution of CC genotype and CG＋GG genotype in cervical vertebra had statistic significance （P=-0.025）. Conclusions Significant difference between C〉G SNP in bFGF gene and spinal fusion through bone graft exists, and C〉G SNP is related to the speed of infusion through bone graft in cervical vertebra in the population of Shandong Peninsula.