多孔性壳聚糖-胶原材料促进神经纤维生长的实验研究

Chitosan-collagen biomaterial promotes the growth of neural fibers of the sciatic nerve

目的比较在体横断性损伤的坐骨神经在壳聚糖-胶原复合管和硅胶管内的生长情况。方法采用冷冻干燥法将壳聚糖及组织胶原制备成壳聚糖-胶原管形支架,切除一侧大鼠坐骨神经1cm,将损伤神经分别显微缝合于壳聚糖-胶原管形支架内作为实验组,缝合至普通硅胶管内作为对照组。术后7周、15周分别做神经纤维的髓鞘特异性染色、髓鞘超微结构观察并用在体坐骨神经复合动作电位直接记录法检测神经纤维生长、髓鞘再生及神经冲动的传导情况。结果术后7周,实验组、对照组的神经两断端之间部位已有较少神经纤维,远离中枢端的复合动作电位不明显。术后15周,实验组的髓鞘化优于对照组,复合动作电位的波幅亦高于对照组。结论多孔性壳聚糖-胶原材料可促进神经纤维生长。

Objective To detect the role of chitosan-collagen in the growth of neural fibers, and compare regeneration of injured sciatic nerves in conduits of chitosan-collagen and silicone. Methods The technique of freeze drying was employed to produce the material conduit of chitosan and collagen. Animal models were established by cutting off sciat- ic nerves in Wistar rats and the injured nerves were bridged by either chitosan-collagen or silicone conduits. 7 weeks and 15 weeks after surgery, morphological and electrophysiological examinations were used to detect the growth of fibers. Results At 7 weeks after surgery, few fibers were detected and the spike potentials were weak in both groups. At 15 weeks after surgery, myelination in the chitosan-collagen group was better than that in the silicone group. Also, the spike potential value was higher than that in the controls. Conclusion Chitosan-collagen material promotes the growth of neural fibers.