RP-HPLC测定糙叶五加不同药用部位中槲皮素和山柰酚

Determination of quercetin and kaempferol in different medicinal parts of Acanthopanax henryi by RP-HPLC

目的建立同时测定糙叶五加不同药用部位中槲皮素和山柰酚的方法。方法采用RP-HPLC法,色谱柱为AT.Lichrom ODS-C18柱(250mm×4.6mm,5μm),以甲醇-磷酸-水(50:0.2:49.8)为流动相,柱温30℃,体积流量1.0mL/min,检测波长360nm,进样量10μL。结果槲皮素在0.018～0.720μg线性关系良好(r=0.9996),加样回收率为98.41%,RSD为0.91%;山柰酚在0.016～0.640μg线性关系良好(r=0.9997),加样回收率为98.06%,RSD为1.39%。槲皮素和山柰酚在叶中的量最高,其次是茎,根中未检出。结论对糙叶五加不同药用部位槲皮素和山柰酚进行了测定,所建立的HPLC法稳定性好、准确性高,适用于糙叶五加中槲皮素和山柰酚的定量分析。

Objective To establish a RP-HPLC method for the determination of quercetin and kaempferol in different medicinal parts of Acanthopanax henryi. Methods The analysis was performed on an AT.Lichrom ODS-C18 column （250 mm×4.6 mm,5 μm） with methanol-phosphoric acid-water （50︰0.2︰49.8） as mobile phase.The flow rate was 1.0 mL/min.The column temperature was 30 ℃, and the detection wavelength was 360 nm. Results The established method showed good linearity for quercetin and kaempferol over the range of 0.018-0.720 μg （r=0.999 6） and 0.016-0.640 μg （r=0.999 7）, respectively. The average recovery of the method for quercetin and kaempferol were 98.41% with the RSD 0.91% and 98.06% with the RSD 1.39%, respectively. The highest content of quercetin and kaempferol was in the leaves of A. henryi, followed by stems, and undetected in roots. Conclusion Contents of quercetin and kaempferol in different medicinal parts of A. henryi are determined. The established HPLC method shows good stability and high accuracy, and can be used for the quantitative analysis of quercetin and kaempferol in A. henryi.