高纯度共轭亚油酸的制备

Research on the Preparation Method of High Purity Conjugated Linoleic Acid

以红花籽油为原料,比较了2种高纯度共轭亚油酸(CLA)的制备方法:(1)碱法异构化-尿素包合、(2)皂化-尿素包合-碱法异构化。针对每种方法确定了尿素包合和碱法异构化的最佳工艺条件,并对所得的CLA异构体组成进行了分析。研究表明:按照方法(2)先获得高纯度亚油酸(LA),再对其碱法异构化制备CLA可以得到纯度更高的产品。该方法在最佳反应条件下,制备得到的CLA的纯度为97.22%,收率为32.08%,其中c9t11-CLA含量为45.14%,t10c12-CLA含量为49.12%。

High purity conjugated linoleic acid（CLA） was prepared by means of urea adduct purification and conjugation by safflower oil.This paper is focused on two different synthesis methods：（1） alkaline catalysis-urea adduction fractionation,（2） saponification-urea adduction fractionation-alkaline catalysis.The processing parameters and the ratio of CLA isomers for each method were investigated.The result showed that the second method which was enriching linoleic acid（LA） first and then performing alkaline catalyzed conjugation was better.Under the optimal conditions,CLA of 97.22% purity was achieved;the total recovery of the product was 32.08%,the content of c9,t11-CLA and t10,c12-CLA were 45.14% and 49.12% respectively.