克鲁斯假丝酵母ZJB 09162产羰基还原酶的产酶条件

Optimization of Culture Conditions for Carbonyl Reductase by Candida krusei ZJB 09162

研究了克鲁斯假丝酵母Candida krusei ZJB 09162产羰基还原酶的条件,确定了最适的培养基组成和培养条件:葡萄糖50.0 g/L,酵母膏50.0 g/L,(NH4)2HPO4 2.5 g/L,KH2PO4 2.5 g/L,NaCl 1.0 g/L,培养基初始pH6.0,装液量12%,接种量4%。将此条件下发酵培养60 h的菌体用于(R)-1,3-丁二醇的不对称合成,产物对映体过量值99%,产率85%。

（R）-1 ,3-butanediol is an important chiral building block and template in asymmetric synthesis. Car- bonyl reductase catalyzed asymmetric reduction of 4-hydroxy-2-butanone is an effective way for large scale production of （R）-1 ,3-butanediol. In this paper, culture conditions of Candida krusei ZJB 09162 producing carbonyl reductase were optimized as follows： glucose 50.0 g/L, yeast extract 50.0 g/L, （ NH4）2HPO4 2.5 g/L, KH2PO4 2.5 g/L, NaCI 1.0 g/L,initial pH 6.0, medium volume 12% , inoculum size 4%. Under these conditions, the yield of （R）- 1,3-butanediol reached 85% , with an optical purity of 99% e.e.