摘要
研究了不同培养基、碳、氮营养对金龟子绿僵菌菌落直径、菌丝生物量、分生孢子产生量及其次生代谢产物对植物病原真菌的抑菌活性。发现金龟子绿僵菌的菌丝线性生长以PSA、麦芽糖和酵母最好,以Czapek、甘露醇和脲最差;菌丝生物量以PSA、白砂糖和蛋白胨最大,以Czapek、乳糖和脲最小;分生孢子产生量以PSA、麦芽糖和KNO3最多,以Czapek、甘油和硫酸铵最少。代谢液的抑菌活性实验结果表明,在不同培养基培养的代谢液中,Czapek的抑菌效果最好,SDAY的抑菌效果最差。在不同碳营养培养的代谢液中,白砂糖的抑菌效果最好,乳糖的抑菌效果最差。在不同氮营养培养的代谢液中,蛋白胨的抑菌效果最好,脲的抑菌效果最差。
The biological characteristics of Metarhizium anisopliae were compared with different culture medici, carbon sources and nitrogen sources by measuring the mycelium growth, dry mycelium biomass and conidia production. The results showed that the optimum culture media, carbon source and nitrogen source for the mycelia growth of M. anisopliae was PSA, maltose and yeast, respectively. The PSA, white granulated sugar and peptone were the suitable media, carbon sources and nitrogen sources for the biomass accumulation, which are in contrast to Czapek, lactose and urea. PSA is suit for the conidial production and the maltose and potassium nitrate was the most feasible car bon source and nitrogen source for conidial compared to glycerol and ammonium sulfate. The anti-fungi assay of the secondary metabolites of M. anisopliae revealed that secondary metabolites produced in Czapek liquid media have more inhibition effects than that in SDAY. Similarly, the anti-fungi effects by the secomtary metabolites produeced in the sucrose as the carbon source and/or the peptone as the nitrogen sources is better than those by others.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2011年第7期51-56,共6页
Food and Fermentation Industries
基金
国家自然科学基金(30972368)
关键词
碳营养
氮营养
金龟子绿僵菌
生物学特性
抑菌活性
carbon nutrition, nitrogen nutrition, Metarhizium anisopliae, biological characteristics, anti-fungal activity