雄激素受体在多囊卵巢综合征卵巢中表达的研究

Study of androgen receptor expression in ovary of polycystic ovary syndrome

目的探讨卵巢组织雄激素受体(androgen receptor,AR)与多囊卵巢综合征(polycystic ovary syndrome,PCOS)发病机理及其临床特征的关系,以期为多囊卵巢综合征的预防和治疗提供理论依据。方法选择因多囊卵巢综合征行卵巢楔形切除的卵巢病理组织15例为病例组,同时选择年龄相匹配的非多囊卵巢综合征并于卵泡期行手术切除的正常卵巢组织13例为对照组。HE染色,采用双盲读片,比较两组中各级卵泡的数目;免疫组织化学染色法检测AR在各级卵泡颗粒细胞中的表达情况。结果两组卵巢组织切片始基卵泡数、闭锁卵泡数比较,差异均无统计学意义;PCOS组初级卵泡数、次级卵泡数、窦卵泡数较对照组增多,差异均有统计学意义(均有P<0.05)。AR在始基卵泡及生长卵泡的卵母细胞表达,在生长卵泡的颗粒细胞表达更明显。结论多囊卵巢综合征患者卵巢局部组织的AR表达与多囊卵巢综合征的发生有一定的相关性。

Objective To explore the correlation of ovarian tissue androgen receptor（AR） with clinical features and pathogenesis of polycystic ovary syndrome（PCOS）,so as to provide theoretical basis for prevention and treatment of PCOS.Methods 15 cases of ovarian wedge resection of ovarian pathology due to PCOS were recruited,13 cases of normal（non-PCOS） ovarian tissue from age-matched patients at early stage of the menstrual cycle were included as control group.The ovarian follicles of two groups were counted by morphometric analysis.The expression of AR was analyzed with immunohistochemical staining.Results The total number of growing follicles was significantly greater in PCOS ovaries than normal,but the number of nongrowing primordial follicles was almost the same.The transitional primary follicles,typical primary follicles,secondary follicles and antral follicle were significantly more than control group（P0.05）.AR was expressed in the oocyte of primordial follicle and preantral follicle,antral follicle.There were stronger intensities immunostaining in the granulosa cells of preantral follicles and antral follicles.Conclusions These is positive correlation between AR expression of ovarian local groups of and PCOS.