煤矿土壤甲烷氧化菌的分离鉴定及其特性

Separation, identification and characterization of methanotrophs in mine soil

根据实验室自行设计方案从淮南潘一矿煤矿土壤中分离出多株能以甲烷为唯一碳源的菌株,挑选其中一株降解甲烷能力较高的菌株H4进行了研究,观察该菌株的菌落、菌体形态,并对该菌株的培养条件和降解甲烷的特性进行研究。结果表明,该菌株以甲烷为碳源生长最好,另外该菌株也可以利用多种形态的氮源,但以硝酸钾和氯化铵共同作用最好,最适培养温度32℃,pH为6.5左右,微量元素Cu2+质量浓度约为15 mg/L。优化培养条件为:32℃,pH 6.5,微量元素Cu2+质量浓度为15 mg/L。

Strain H4,one of some methanotrophic bacteria isolated from soil of Huainan samples utilizing the separate device designed by our laboratory,has been studied in this paper.The culturing and reaction conditions for methane utilization of this strain were investigated.The results indicated that the optimal conditions for the growth of strain H4 were methane gas as carbon source,and the strain can also use a variety of forms of nitrogen,but the interaction of potassium nitrate and ammonium chloride the best.And its cultivation temperature is 32 ℃ and the medium initial pH is 6.5,and its optimal Cu2＋ mass concentration is 15 mg/L.As well as the optimal culture conditions are： 32 ℃,pH 6.5,and Cu2＋ mass concentration of 15 mg/L.