吸烟所致肺气肿模型大鼠戒烟后肺病理及炎性介质的变化

The lung pathology changes and the expression of inflammatory mediators in smoking induced rats of emphysema model after smoking cessation

目的探讨吸烟所致肺气肿模型大鼠在戒烟后肺组织病理及炎性介质表达变化规律。方法选用Wistar雄性大鼠80只,随机分为非吸烟正常对照组及吸烟所致肺气肿模型戒烟0 d、1 w、2 w、4 w、6 w、8 w、12 w组。采用酶联免疫吸附方法测定各组大鼠血清中IL-8的蛋白质含量,S-P免疫组化学方法检测肺组织NF-κB p65的表达,并光镜观察HE染色切片、对大鼠气道炎症进行病理学评分。结果肺气肿大鼠模型成功复制:肺组织HE染色切片,光镜下观察,其病理变化与人类肺气肿相似;肺气肿各时相戒烟组大鼠血清IL-8浓度、肺组织NF-κB的表达及病理评分较未吸烟正常对照组明显升高(P<0.05);肺气肿各相邻时相戒烟组间比较,各检测指标有上升趋势,戒烟12 w组大鼠血清中IL-8的蛋白质含量、肺组织NF-κB p65的表达、气道炎症的病理学评分均较戒烟0天组明显升高(P<0.05)。结论吸烟所致肺气肿模型大鼠形成后,其气道炎症在戒烟后12周内仍可见持续加重。

Objective To investigate the lung pathology changes and the expression of inflammatory mediators in smoking induced rats of emphysema model after smoking cessation.Methods 80 Wistar male rats were randomly divided into control and smoking induced emphysema model groups on the smoking cessation stage 0 day,1,2,4,6,8 and 12 weeks.Levels of IL-8 in serum was detected by ELISA,expression of NF-κB p65 in lung tissue was detected by S-P immunohistochemistry,and pathology score of inflammation on airway was carried out by observing HE stained lung tissue sections under light microscope.Results Rats of emphysema model was successful replicated： the pathological changes were similar to human emphysema by observing HE stained lung tissue sections under light microscope.Levels of IL-8 in serum,expression of NF-κB and pathological score in lung tissue of all emphysema model groups on any smoking cessation stage were significantly higher than those of non-smoking control group（P0.05）.All indexes between adjacent stage emphysema groups had an upward trend.Levels of IL-8 in serum,expression of NF-κB and airway inflammation pathological score of groups on smoking cessation stage 12 weeks were significantly higher than those of groups on smoking cessation stage 0 days（P0.05）.Conclusions After the rats of emphysema model is formed,their airway inflammation is still visible and continue to increase after 12 weeks smoking cessation.