补体3、补体4B1及载脂蛋白E在胰腺癌中的表达及临床意义

The expressions and clinical significance of complements 3, 4BI and apolipoprotein E in pancreatic cancer

目的探讨补体3（C3）、补体4BJ（C481）、载脂蛋白（Apo）E在胰腺癌中的表达及其与TNM分期和胰腺癌淋巴转移的相关性。方法收集胰腺癌病理切片标本38例及胰腺癌新鲜标本和癌旁正常胰腺组织各20例。分别应用免疫组织化学和Western印迹方法检测胰腺癌和正常胰腺组织中C3、C481、ApoE的表达情况，观察C3、C481、ApoE阳性表达率和灰度比的差异。使用SPSS13．0统计软件包分析其与胰腺癌TNM分期和淋巴转移的相关性。结果38例胰腺癌组织中C3、C481、ApoE表达率分别为73．68％（28／38）、86．84％（29／38）、76．31％（33／38），高于正常胰腺组织的42．11％（16／38）、26．32％（10／38）、42．11％（16／38），差异均有统计学意义（x^2分别=7．77、19．01、16．60，P值分别=0．01、0．00、0．00）。C3、C481、ApoE在胰腺癌中的灰度比分别为1．63±0．28、1．25±0．18、2．57±0．22，高于正常胰腺（0．88±0．19、0．65±0．13、1．28±0．24），差异均有统计学意义（x^2值分别=9．93、11．81、17．71，P值均=0．00）。C3与胰腺癌的TNM分期和淋巴转移无关。C481、ApoE与TNM分期和淋巴转移密切相关。Ⅱ至Ⅳ期胰腺癌和有淋巴转移胰腺癌中C481、ApoE表达明显高于I期胰腺癌和无淋巴转移胰腺癌。结论C3、C481、ApoE在胰腺癌中均呈高表达。C3只参与胰腺癌的早期过程，与胰腺癌发展进程无关。C481、ApoE参与胰腺癌生长和转移。

Objective To investigate the expressions of complements 3 （C3）, 4B1 （C4B1） and apolipoprotein E （ApoE） in pancreatic cancer and relations with TNM staging and lymph node metastasis of pancreatic cancer. Methods Thirty-eight pancreatic cancer biopsy specimens, 20 fresh pancreatic cancer specimens and 20 adjacent normal tissues of pancreatic cancer were collected. The expressions of C3, C4B1, ApoE in pancreatic cancers and normal pancreatic tissues were detected by immunohistochemistry and Western-Blot, the positive expression rates of C3, C4B1, ApoE and the differences in gray scale were also observed. Their association with pancreatic cancer TNM staging and lymph node metastasis were analyzed by SPSS 13. 0. Results The expression rates of C3, C4B1, ApoE in pancreatic cancer were 73. 68% （28/38）, 86. 84% （29/38） and 76. 31% （33/38） respectively, higher than those in normal pancreatic tissues which were 42.11% （16/38）, 26.32% （10/38） and 42.11% （16/38） accordingly, the differences were statistically significant （x^2 was 7.77, 19.01, 16.6, and P value were 0. 01, 0. 00, 0. 00 respectively）. The gray scale of C3, C4B1 and ApoEin pancreatic cancer were 1. 63±0, 28,1.25±0. 18 and 2. 57±0. 22 respectively, higher than those in normal pancreatic tissue （0.88±0.19,0.65 ± 0.13,1.28 ± 0.24 respectively）, the differences were statistically significant （t value were 9. 93, 11. 81, 17. 71 and all P value were 0.00, respectively）. There was no association between C3 and TNM staging or lymphatic metastasis of pancreatic cancer. C4B1 and ApoE were closely related with TNM stage and lymph node metastases. The expressions of C4B1 and ApoE in stage 1I to IV pancreatic cancer or with lymphatic metastasis were significantly higher than those in stage I pancreatic cancer and those without lymph node metastasis. Conclusion C3, C4B1 and ApoE were all highly expressed in pancreatic cancer. C3 was only involved in early event in pancreatic cancer, not related with development of pancreatic cancer. C4131 and ApoE were involved in tumor growth and metastasis.