摘要
目的通过探索D-聚甘酯抗凝活性与Ⅹ／Ⅱ因子灭活度比值的相关性，推断其可能的抗凝抗栓机制，为临床更加合理应用该药提供参考。方法设4个不同D．聚甘酯剂量组以及空白、肝素钠2个对照组，每组均测定凝血酶原时间（PT），凝血活酶时间（APTT）以及Ⅱ因子、Ⅹ因子的活性，并计算Ⅹ／Ⅱ的灭活度比值。结果在50-150μg·mL-1内，随着D-聚甘酯浓度的增加，其抗凝活性增强，Ⅹ／Ⅱ因子灭活度比值升高。结论D-聚甘酯在发挥抗凝活性时，可能的机制是以灭活凝血酶活性为主，而不是以抑制凝血酶原产生为主。
OBJECTIVE To investigate relationship between anticoagulation and deactivation ratio on Ⅹ/Ⅱ factor of D-polymannuronicate and estimate the anticoagulation mechanism. METHODS Plasma sample was divided into 6 groups： four D-polymannuronicate groups in different doses, one blank group and one heparin sodium group. APTT, PT and the activity of factor Ⅹ/Ⅱ in each group were determined, then the deactivation ratio on Ⅹ/Ⅱ factor was calculated. RESULTS The anticoagulation as well as the deactivation ratio on Ⅹ/Ⅱ factor was enhanced with the increased concentration of D-polymannuronicate of 50-150 μg·mL-1. CONCLUSION The possible anticoagulation mechanism of D-polymannuronicate is deactivating the activity of thrombin rather than inhibiting thrombinogen.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2011年第8期694-696,共3页
Chinese Journal of Modern Applied Pharmacy
基金
中国海洋大学海洋药物教育部重点实验室开放基金资助项目[KLMD(OUC)200808]
关键词
D-聚甘酯
抗凝活性
灭活度比值
相关性
D-polymannuronicate
anticoagulation
deactivation ratio
relationship
