回归正交设计优化西红花苷的提取工艺

Optimization of Extraction of Crocin from Crocus Sativus L.by Orthogonal Regression Design

目的研究从西红花中提取西红花苷的最佳工艺条件。方法以西红花苷的提取率为指标,在使用单因素试验方法考察乙醇浓度、提取时间、提取温度对西红花中西红花苷提取率的基础上,应用回归正交方法建立3元2次方程优化西红花苷的提取工艺。结果最佳的提取工艺是:乙醇浓度52%,提取时间30 min,提取温度30℃。此条件下,提取率为0.643 5 mg.g?1。结论在优化的提取工艺下,西红花中西红花苷提取率较高。

OBJECTIVE To study the optimal extraction condition of crocin from Crocus sativus L. METHODS Taking the total extraction ratio of crocin from Crocus sativus L. as an index, the quadratic orthogonal regression design which was based on the single factor method including the concentration of ethanol, extraction time and extraction temperature was employed to build the equation and to optimize the extraction technique. RESULTS The optimized condition was as follows： the concentration of ethanol was 0.52%, extracted 30 minutes at the temperature of 30℃, and the extraction ratio reached 0.643 5 mg·g-1. CONCLUSION Under the optimal extraction condition, the total extraction ratio of crocin from Crocus sativus L. is much higher.