均匀设计法优化人乳中甲硝唑的检测

Optimize the Determination of Metronidazole in Human Milk with Uniform Design Method

目的优化人乳中甲硝唑的检测,建立限度检查方法。方法用均匀设计法确定试验方案,优化从人乳中提取甲硝唑的条件,用HPLC测定甲硝唑。结果从人乳中提取甲硝唑,不需要加氢氧化钠,也可以不进行超声处理。限量检测方法的验证试验结果,空白样品色谱图上在与供试品和对照品色谱相应位置处没有色谱峰;检测限<0.02μg·L-1;在不同条件下检测同一样品,结果RSD为2.2%。结论用均匀设计法可以迅速有效地确定检测方法的前处理条件。建立的人乳中甲硝唑限度检测方法灵敏度高、操作简单、分析速度快、重复性好。

OBJECTIVE To optimize the determination of metronidazole in human milk and establish a limit test method. METHODS The experiments scheme was scheduled by uniform design method to improve the extraction of metronidazole from human milk, and the metronidazole was determined by HPLC method. RESULTS For extracting metronidazole from milk, the NaOH should not be added and the super sonic treatment could be removed. In the results of the validation experiments of the limit test method, there was no corresponding peak in the chromatograph of the control comparing with the chromatograph of the sample and the standard, the limit of detection was lower than 0.02 pg-L^1, and the RSD of the test results of the same sample in different conditions was 2.2%. CONCLUSION Uniform design method is effective and rapid to improve the parameters of the test method. The limit test method of metronidazole in human milk established is sensitive, simple, rapid and reproducible.