胶质瘤1p/19q联合缺失特征分析

Analysis of 1p/19q Codeletion in Gliomas:Report of 63 Cases

目的研究胶质瘤中1p/19q联合缺失与患者临床特征及O6-甲基鸟嘌呤-DNA甲基转移酶(O6-methylguanine-DNA methyltransferase,MGMT)表达的关系。方法使用荧光原位杂交技术,对中山大学附属肿瘤医院2009年3月至2011年3月的63例手术确诊胶质瘤标本进行1p/19q联合缺失检测,利用统计学方法分析1p/19q联合缺失患者与无联合缺失患者在性别、年龄、部位、病理类型及MGMT表达状态是否有差异。结果全组63例患者,男38例,女25例,年龄(41.7±15.3)岁。其中,16例(25.4%)存在1p/19q联合缺失。1p/19q联合缺失患者与无联合缺失患者在性别、年龄及肿瘤部位间的差异无统计学意义。各病理类型1p/19q联合缺失的比例从高到低依次为少突胶质细胞瘤(9/16)、间变性少突胶质细胞瘤(3/8)、星形细胞瘤(2/10)、间变性星形细胞瘤(1/6)及胶质母细胞瘤(1/21),其差异有统计学意义(P<0.01)。在全组63例胶质瘤及25例含少突成分胶质瘤中,MGMT阳性肿瘤1p/19q联合缺失比例与MGMT阴性组间的差异皆无统计学意义。结论 1p/19q联合缺失主要与胶质瘤的病理类型有关,含少突成分胶质瘤中1p/19q联合缺失比例较高。1p/19q联合缺失可作为少突胶质细胞瘤病理诊断的重要参考指标。

Objective To detect 1p/19q codeletion in patients with gliomas and investigate the relationship between 1p/19q codeletion and other clinical features including age, sex, location of tumors and the expression of 06- methylguanine-DNA methyhransferase （MGMT）. Methods Fluorescence in situ hybridization （FISH） was used to detect 1p/19q codeletion in 63 cases with pathologically confirmed gliomas in Sun Yat-sen University from March 2009 to March. Clinical data were retrospectively reviewed. The relationship between 1p/19q codeletion and other clinical features including age, sex, location of tumors and the expression of MGMT was analyzed. Results Among 63 patients, 38 were male and 25 were female. The mean age of patients was 41.7 years. Codeltion of 1p/19q was detected in 16 cases （25.4%）. There were no statistical differences in sex, age and tumor location between glioma patients with and without 1p/19q codeletion. Codeletion of 1p/19q, in an descend order, was detected in oligodendrogliomas （9/16）, anaplastic oligodendrogliomas （3/8）, astrocytomas （2/10）, anaplastic astrocytomas （1/6） and glioblastoma muhiforme （1/21）. There were statistical differences in the distribution of 1p/19q in different pathological types （P〈 0.01 ）. Among 63 cases, 34 were MGMT- positive and 29 were MGMT-negative. No statistical differences of status of 1p and 19q codeletion were found between MGMT-positive and-negative tumors. Conclusions 1p/19q codeletion is associated with oligodendroglial tumors and is a useful marker for pathological diagnosis.