高效液相色谱法测定化妆品中的12种紫外吸收剂

Determination of 12 sunscreen agents in cosmetics by high performance liquid chromatography

建立了高效液相色谱同时测定化妆品中12种紫外吸收剂含量的方法。样品用甲醇提取,高速离心,过滤,以SB-C8柱(250mm×4.6mm,5μm)为分离色谱柱,甲醇和0.1%(v/v)甲酸水溶液为流动相,梯度洗脱,以311nm为检测波长进行定性、定量分析。该方法前处理简单、易操作,12种紫外吸收剂分离效果良好;在1.0～500mg/L范围内呈线性关系,相关系数大于0.9995;方法检出限为0.002～0.1mg/L;实际样品中的加标回收率为97.4%～107.5%,相对标准偏差为1.54%～4.98%。该方法简便、准确,能够满足化妆品中紫外吸收剂的检测要求。

A comprehensive analytical method based on high performance liquid chromatography（HPLC） has been developed for the determination of 12 sunscreen agents in cosmetics.The cosmetic samples were extracted by methanol.The target compounds were separated on an SB-C8 column（250 mm×4.6 mm,5 μm） in gradient elution mode using methanol and 0.1%（v/v） formic acid aqueous solution as mobile phases.The detective wavelength was 311 nm.The linear plots were obtained between 1.0 and 500 mg/L with good correlation coefficients larger than 0.999 5.The limits of detection（LODs） for this method were in the range of 0.002-0.1 mg/L.The spiked recoveries of commercial cosmetics were in the range of 97.4%-107.5% with the relative standard deviations of 1.54%-4.98%.The results indicated that the developed method is simple,rapid,accurate and suitable for the determination of 12 sunscreen agents in cosmetics samples.