昆明山海棠片所致大鼠睾丸损伤的病理学研究

Pathological Study of Testicular Injury Induced by Tripterygium hypoglaucum Hutch(THH) in Rats

目的:观察昆明山海棠片连续给药90 d对大鼠睾丸结构的损伤。方法:80只SD雄性大鼠随机分为5.0 g/kg、10.0 g/kg、20.0 g/kg 3个给药组及正常对照组(等量蒸馏水),连续灌胃3个月。于给药1个月、2个月、3个月,采用光学显微镜、透射电子显微镜观察睾丸组织形态学的变化。结果:各给药组均可引起大鼠生精细胞损伤和破坏;睾丸超微结构的改变主要包括各级生精细胞坏死、空泡化,细胞质内溶酶体数量增多,线粒体肿胀、固缩,内质网扩张,细胞核染色质异常分布、溶解、固缩,核崩解消失等;支持细胞、间质细胞未见明显异常。结论:昆明山海棠片连续给药主要导致大鼠睾丸生精细胞超微结构损伤,其损伤存在剂量效应和时间效应关系。

Objective： To investigate the testicular structural injury induced by Tripterygium hypoglaucum Hutch（THH） in rats.Methods： Eighty male SD rats were randomly divided into 4 groups.Animals were administered with THH at the dose of 0 g/kg（the control,n=20）,5 g/kg（low dose group,n=20）,10 g/kg（modoated dose group,n=20）,20 g/kg（high dose group,n=20） by intragastric administration consecutively for 3 months.Light microscope and transinssion electron microscope were used to observe changes of testicular histomorphology at the 1,2,3 months,resepectively.Results： Injury of testicular spermatogenic cells in rats was induced by ever dosage.The main ultrastructure changes of testis were included necrosis of spermatogenic cells at all levels necrosis and vacuolar degeneration;increasing number of lysosome within cytoplasm;mitochondrion swollen and pyknosis;endoplasm reticulum dilation;abnormal distribution of nuclear chromatin,dissolution and pyknosis degeneration of the nucleus and so on.Sertoli cells and mesenchymal cells were as normal.Conclusion： THH can cause testicular androgone ultrastructural injury in rats,which has a positive correlation with dosage effect and time effect.