壳聚糖固定化木聚糖酶的工艺研究

Study on the Immobilization Xylanase from Aspergillus niger on Chitosan

先对影响固定化木聚糖酶效果的几个单因素如壳聚糖质量浓度、戊二醛体积分数、给酶量等指标进行测定,然后采用L9(43)正交试验设计法对壳聚糖作为载体固定化木聚糖酶的工艺进行优化。单因素试验结果表明,当壳聚糖质量浓度为50和60 g/L时,其固定化酶活力较高;而交联剂戊二醛为60 mL/L时,所形成的载体与酶结合量达最大;将酶液浓缩或稀释一定体积时,浓缩1倍时固定化效果较好。在4因素3水平正交设计中,分别对交联剂和壳聚糖质量浓度、固定化温度及时间进行比较。结果表明,交联剂戊二醛体积分数和壳聚糖质量浓度分别为第1、第2主因素,对固定化酶活影响极显著,固定化温度对固定化酶活影响显著,而固定化时间对固定化酶活影响不显著。通过正交试验,壳聚糖固定化木聚糖酶的最佳工艺为60 mL/L戊二醛、50 g/L壳聚糖、固定化温度30℃、固定化时间3 h。

To optimize the preparation of chitosan microsphere with single factor bolting and orthogona test design,relative activity of immobilized enzymes were studied,xylanase is immobilized on chitosan microsphere by cross linking and adsorbing and the conditions were gained by SPSS software dealing with the date.The single factor experiments showed that activity of immobilized xylanase is better under the condition of 50 g/L and 60 g/L chitosan;the binding capacity between vector and enzyme is obtained at 60 mL/L glutaraldehyde;activity of immobilized enzymes is high if xylanase is concentrated 1 time.The results of orthogona design showed that relative activity of enzyme of immobilized enzymes could be ideal under the conditions of 60 mL/L glutaricialdehyde and 50 g/L chitosan,and immobilized xylanase maintained at 30 ℃ for 3 h.