CdSe/巯基-β-环糊精量子点的纯化及应用

PURIFICATION AND APPLICATION OF CdSe/MERCAPTO-β-CYCLODEXTRIN QUANTUM DOTS

以单-(6-巯基)-β-环糊精作为修饰剂,在水溶液中合成了稳定的CdSe量子点(CdSe/CD QD s),并系统考察了溶剂的种类及加入量对量子点纯化效果的影响.同时研究了该量子点在2种细菌标记及分子识别的应用.结果表明:2倍体积的乙腈完全沉淀CdSe/CD QD s所用的时间最短,纯化后荧光强度略有增强.CdSe/CD QD s能够标记大肠杆菌和枯草杆菌,且能定量检测维生素B1、维生素C及6-巯基嘌呤、6-巯基嘌呤核苷、4-氨基-6羟基-2巯基嘧啶3种杂环小分子.

CdSe quantum dots（QDs） were synthesized in aqueous solution using mono-（6-mercapto）-β-cyclodextrin as stabilizer in this paper.And the purification of CdSe/CD QDs with different solvents in a series of proportions was investigated systematically.The fluorescence properties of CdSe/CD QDs were characterized by fluorescence microscope and fluorescence spectroscopy.In addition,the application of CdSe/CD QDs in bacteria marking and molecular recognition was also studied.The results showed that the purification with twice volume acetonitrile was the best.And CdSe/CD QDs can be used not only to mark E.coli and Bacillus subtilis,but also quantitatively detect vitamin B1,vitamin C,6-mercaptopurine,6-mercaptopurine ribonucleoside,and 4-amino-6-hydroxy-2-mercaptoprimidine.