虎尾兰组培快繁技术研究

Study on Technology for Tissue Culture and Rapid Propagation of Sansevieria trifasciata L.

[目的]寻求虎尾兰组培快繁的最佳培养基。[方法]以虎尾兰幼叶为外植体,采用2种不同浓度的消毒剂进行处理,探讨外植体最佳消毒方法。以MS为基本培养基,研究添加不同浓度的2,4-D、6-BA和NAA对虎尾兰幼叶愈伤组织诱导、芽分化诱导及生根的影响,确定虎尾兰组培快繁的最佳培养基。[结果]虎尾兰外植体最佳消毒方式为70%酒精10 s+0.1%升汞5 min,外植体污染率为7.7%;虎尾兰愈伤组织诱导最适宜培养基为MS+0.25 mg/L 2,4-D,出愈率为100%;诱导芽分化的最适宜培养基为MS+0.5 mg/L 2,4-D+2.0 mg/L 6-BA,分化率为76%;根培养的最适宜培养基为MS+4.0 mg/L NAA,生根率达到100%,试管苗成活率达95%。[结论]该研究为虎尾兰组培快繁的大规模工厂化生产提供了科学依据。

[Objective] The study aimed to seek for the optimum medium of tissue culture and rapid propagation of Sansevieria trifasciata L.[Method] With the young leave of S.trifasciata as the explants,they were treated by 2 kinds of sanitizers with different concn.so as to discuss the optimum sterilizing method.With MS as the basic medium,the effects of adding 2,4-D,6-BA and NAA with different concn.on the induction of S.trifasciata callus and bud differentiation and the their rooting so as to confirm the optimum medium of tissue culture and rapid propagation of S.trifasciata.[Result] The optimum sterilizing method for the explants of S.trifasciata was as follows： 70% ethanol adding 0.1% HgCl2 was chosen to sterilize for 5 min,which could decreased the pollution rate of explants,being only 7.7%.The optimum medium for the callus induction of S.trifasciata was MS＋0.25 mg/L 2,4-D with the induction rate of 100%,that for its bud differentiation was MS＋0.5 mg/L 2,4-D ＋2.0 mg/L 6-BA with the bud differentiation rate of 76% and that for rooting was MS＋4.0 mg/L NAA with the rooting rate of 100% and the survival rate of seedlings in the test tubes was up to 95%.[Conclusion] The research provided the scientific basis for the large-scale factory production of the tissue culture and rapid propagation of S.trifasciata.