三角梅外植体消毒和愈伤组织诱导研究

Study on the Disinfection and Callus Induction of the Explants of Bougainvillea glabra

[目的]探索三角梅的最佳消毒时间和最佳愈伤组织诱导培养基。[方法]采用不同消毒时间对三角梅的7种外植体进行灭菌,并用不同激素与浓度组合的培养基诱导愈伤。[结果]三角梅的最佳外植体为半木质化茎段;最佳消毒方法为先用浓度75%酒精溶液灭菌30 s,无菌水冲洗5次,再用浓度0.1%升汞溶液消毒9 min,最后用无菌水冲洗7~8次;最佳诱导培养基为MS＋2.0 mg/L 6-BA＋1.0 mg/L NAA。[结论]获得了三角梅外植体的最佳消毒方式和最佳愈伤组织诱导培养基,为三角梅组织培养体系的建立奠定了基础。

[Objective] To explore the best disinfection time and the best callus induction medium of Bougainvillea glabra.[Method] 7 explants of Bougainvillea glabra were disinfected for different time,and the callus was induced with different hormone and concentration combinations.[Results] The optimal explant of Bougainvillea glabra was semi-woody stem;the best disinfection condition was 75% alcohol sterilized for 30s,aseptic water flushed 5 times,then 0.1% liters of mercury disinfected for 9 minutes,finally aseptic water flushed 7-8 times;the best induction culture medium was MS＋2.0 mg/L 6-BA＋1.0 mg/L NAA.[Conclusions] The best disinfection time and the best inducing culture medium of the explants of Bougainvillea glabra were obtained,which laid the foundation for the establishment of tissue culture system of Bougainvillea glabra in the future.