人参及其混伪品的紫外谱线组法鉴别研究

Study on the Identification of RADIX GINSENG and Its Counterfeits by UASLG Method

[目的]建立鉴别人参及其混伪品桔梗、板蓝根和北沙参的紫外谱线组鉴别方法。[方法]采用水、乙醇、石油醚、乙酸乙酯和氯仿对4种药材分别进行提取,然后进行紫外检测及其一阶导数光谱检测。[结果]正品人参与桔梗、板蓝根和北沙参的紫外吸收光谱的峰位置和吸光度有明显的差异。[结论]该方法简便、准确、灵敏,能够很好的区别人参及其混伪品。

[Objective] To establish a method for distinguishing RADIX GINSENG from its counterfeits including RADIX PLATYCODI,RADIX ISATIDIS and RADIX GLEHNIAE by ultraviolet absorption spectra Lines group（UASLG）.[Method] The 4 kinds of medicines were extracted with five different solvents of water,ethanol,petroleum ether,ethyl acetate and chloroform.Then,their absorbance in UV and one-order derivative spectra was measured.[Result] There were obvious differences regarding peak position and absorbance in ultraviolet absorption spectra among RADIX GINSENG and RADIX PLATYCODI,RADIX ISATIDIS and RADIX GLEHNIAE.[Conclusion]The method was simple and accurate,and could be used to distinguish RADIX GINSENG from its counterfeits.