车前子乙酸乙酯部位化学成分HPLC指纹图谱研究

Study on the HPLC Fingerprint of Chemical Constituents in Ethyl Acetate Parts of SEMEN PLANTAGINIS

[目的]建立车前子乙酸乙酯部位的化学成分指纹图谱,为车前子的质量控制及品种鉴定提供依据。[方法]色谱条件为:Diamon-sil C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.5%的乙酸水溶液,流速为1.0 ml/min,检测波长为270 nm,采用梯度洗脱。[结果]江西道地产区10批车前子乙酸乙酯部位化学成分指纹图谱共检出12个分离度良好的共有峰。不同品种车前子乙酸乙酯部位指纹图谱相似度相差很大。[结论]该方法简便、快速,可作为车前子药材的质量控制和品种鉴定的有力手段。

[Objective] To establish the fingerprint of chemical constituents of ethyl acetate parts by HPLC,so as to provide a basis for quality control and variety identification of SEMEN PLANTAGINIS.[Method] The chromatographic separation was performed on a C18 column（250 mm×4.6 mm,5 μm） using the mixture of acetonitrile-0.5% acetic acid aqueous solution as mobile phase in gradient elution.The flow rate was 1.0 ml/min,and the detection wavelength was 270 nm.[Result] There were 12 common peaks in the HPLC fingerprint of chemical constituents of ethyl acetate parts of 10 batches of SEMEN PLANTAGINI produced in Jiangxi authentic regions.Fingerprint similarity of ethyl acetate parts of SEMEN PLANTAGINI varied obviously for different varieties.[Conclusion] The HPLC fingerprint method was simple,rapid and feasible in the quality control and varieties identification of SEMEN PLANTAGINI.