丁香·厚朴不同溶剂萃取物的抑菌性和抗氧化性研究

Study on the Antimicrobial and Antioxidative Activities of Different Extracts from FLOS CARYOPHYLLI and CORTEX MAGNOLIAE OFFICINALIS

[目的]研究丁香、厚朴不同溶剂萃取物的抑菌性和抗氧化性。[方法]利用石油醚、乙酸乙酯对丁香和厚朴的醇提物进行萃取,以食品中常见的8种腐败菌为试验菌种,采用纸片法研究各萃取相的抑菌性;用清除二苯代苦味肼基自由基(DPPH)法和Rancimat法研究各萃取相的抗氧化性。[结果]丁香的石油醚萃取液、厚朴的石油醚萃取液、乙酸乙酯萃取液对食品腐败常见的8种菌有较强的抑菌效果。各萃取液对DPPH自由基基均有较强的清除作用,但丁香的各萃取相提取物清除自由基能力均强于厚朴萃取相。各萃取液对猪油体系抗氧化能力的强弱依次为:厚朴乙酸乙酯萃取液>厚朴石油醚萃取液>丁香乙酸乙酯萃取液>丁香石油醚萃取液>丁香水萃取液>厚朴水萃取液。[结论]该研究为开发天然食品防腐剂和抗氧化剂提供了科学依据。

[Objective] To study the antimicrobial and antioxidative activities of the different extracts from FLOS CARYOPHYLLI and CORTEX MAGNOLIAE OFFICINALIS.[Method] The ethanol extract from FLOS CARYOPHYLLI and CORTEX MAGNOLIAE OFFICINALIS were respectively extracted by petroleum ether and ethyl acetate.Eight common microbial strains were used to evaluate the antimicrobial activity of the extracts by filter paper method and the antioxidative activity of the extracts was determined respectively by DPPH free radical elimination method and Rancimat method.[Result] The petroleum ether extracts of FLOS CARYOPHYLLI and petroleum ether and ethyl acetate extracts from CORTEX MAGNOLIAE OFFICINALIS showed strong inhibitory effect to all the tested strains.The scavenging rate of FLOS CARYOPHYLLI extracts to DPPH was stronger than that of CORTEX MAGNOLIAE OFFICINALIS extracts.The antioxidative activities of all extracts in lard system decreased in sequence of CORTEX MAGNOLIAE OFFICINALIS ethyl acetate extract,CORTEX MAGNOLIAE OFFICINALIS petroleum ether extract,FLOS CARYOPHYLLI ethyl acetate extract,FLOS CARYOPHYLLI petroleum ether extract,FLOS CARYOPHYLLI water extract and CORTEX MAGNOLIAE OFFICINALIS water extract.[Conclusion]The study provided scientific basis for exploring natural food preservatives and antioxidants.