金顶侧耳多糖体内免疫活性的初步研究

Preliminary Study on Immunocompetence of Polysaccharides from Pleurotus citrinopileatus Sing

[目的]探究金顶侧耳多糖提取方法及对动物机体免疫功能的影响。[方法]通过液体培养金顶侧耳,以乙醇沉淀法从发酵液中提取金顶侧耳多糖,并采用蒽酮比色法和3,5-二硝基水杨酸法相结合测定多糖的得率。通过称量胸腺、脾脏的重量,计算胸腺指数和脾脏指数来探讨不同剂量的金顶侧耳多糖对小鼠免疫功能的影响。[结果]该法提取的金顶侧耳多糖得率为14.35%,随金顶侧耳多糖的剂量的增加,小鼠脾脏重量增加,注射多糖的小鼠免疫力明显高于环磷酰胺(CY)对照组,低于生理盐水对照组。[结论]金顶侧耳多糖能对CY有减毒作用,能够增强小鼠免疫功能。

[Objective] To study the extraction method of polysaccharides from Pleurotus citrinopileatus Sing and its effects on immune function in animals.[Methods] Through the liquid culture,the polysaccharides were extracted from fermentation broth of Pleurotus citrinopileatus Sing by ethanol precipitation method,whose extraction rate was determined by combining anthrone colorimetry technology with 3,5-dinitrosalicylic acid method.Effects of different doses of polysaccharides from Pleurotus citrinopileatus Sing on immune function in mice were explored by weighing thymus and spleen and calculating thymus index and spleen index.[Results]The extraction rate of polysaccharides was 14.35%.With the dose of polysaccharides increasing,spleen weight gradually increased.The immunity in mice that were injected with polysaccharides was significantly higher than that were injected with cyclophosphamide（CY）,and lower than that were injected with normal saline（NS）.[Conclusion] The polysaccharides from Pleurotus citrinopileatus Sing could play a role in CY attenuation and enhancing immune function in mice.