大鼠背根神经节神经元细胞纯化培养的模型建立

MODEL BULLDING OF PRIMARY CULTURE AND PURIFICATION OF RAT DORSAL ROOT GANGLION NEURONS

目的:建立一种切实可行的新生SD大鼠背根神经节神经元培养及纯化方法。方法:用显微解剖方法获取足够数量新生大鼠背根神经节,通过胰蛋白酶+EDTA消化、交替使用DF-12培养基和加有阿糖胞苷抗有丝分裂的DF-12培养基培养等方法,在体外获得纯化的背根神经节神经元,并采用NSE免疫细胞化学染色方法检测神经元的纯度。结果:获得的背根神经节神经元在体外生长良好,纯度可达到90%以上。结论:本方法可以获得大量高度纯化的大鼠背根神经节神经元。

Objective： To establish a feasible method of culture and purification of newborn dorsal root ganglion neurons of rats. Methods ： Sufficient dorsal root ganglions （DRG） were obtained by microdissection, then were dissociated with trypsin and EDTA. The dissociated neurons were cultured with DF-12 media and Cytarabine for purification. NSE staining was used to determine the purification percentage of DRG. Results： The isolated and cultured DRG survived and grew in good condition in vitro, and the purified percentage of DRG was over 90%. Conclusion： The method is very feasible in obtaining the massive highly purified newborn dorsal root ganglion neurons of rats.