小球藻培养条件及其抑制α-葡萄糖苷酶活性的研究

Study on the culture conditions chlorella spp and its inhibitory activity onα-glucosidase

通过对小球藻培养条件中初始pH值、接种量、培养温度、光照等因素进行研究,探讨小球藻的最佳培养条件,并对培养的小球藻提取物进行α-葡萄糖苷酶活性抑制实验。结果表明,初始pH值为7,接种量为100:10,培养温度为25℃/20℃(昼/夜),光照强度为10000Lx为小球藻的最佳培养条件;其水提物对α-葡萄糖苷酶活性的IC50值为3.14mg/mL,具有一定的抑制活性。

The culture conditions of Chlorella spp such as initial pH,inoculation amount,culture temperature, intensity of illumination were studied.The optimal culture conditions of chlorella spp were that initial pH was 7,inoculation amount was 100：10,culture temperature was 25℃/20℃（day/night）,the intensity of illumination was 10000Lx.In addition,the inhibitory activity onα-glucosidase of the water extract was also studied, and IC_（50） value was 3.14mg/mL.