中草药提取物复配对烟草黑胫病菌抑制作用增效组合筛选

Screening of combined Chinese herb extracts for the fungi toxicity against Phytophthora var.nicotianae

用菌丝生长速率法测定苦参(A)、金银花(B)、黄岑(E)和补骨脂(F)4种中草药提取物对烟草黑胫病菌的抑制作用,建立了毒力回归方程。结果显示,4种药剂的EC50值分别为4.56、5.99、2.65、4.76 mL/L。根据共毒因子法判定4种药剂两两复配后的联合作用,结果表明,苦参+金银花、苦参+补骨脂和金银花+补骨脂组合抑菌增效作用明显,提取物复配后对病菌的抑制存在协同增效作用,复配物抑菌效果好于单剂,并筛选出了各组合的最佳配比。

The inhibitory effect of the four Chinese medicinal herb extract,which include Sophora flavescens（A）,Lonicera japonica（B）,Scutellaria chihshuiensis（E） and Psoralea corylifolia（F）,against Phytophthora var.nicotianae were assayed,by applying Hypha Growth rate method.Then toxicity regression equation was obtained.EC50 value of four herbal extracts were 4.56、5.99、2.65、4.76 mL/L respectively.The synergistic effect of pairwise compounds of the four herbal extracts was determined according to the method of co-toxicity factor.The result showed that compounds of A and B,A and F,B and F,have obvious antibacterial effect.It finds that the inhibitory effect of the compounds of the four herbal extracts against Phytophthora var.nicotianae was synergitic.The inhibitory effect of the compounds was superior to that of simple dosage.And the best ratio of compounds was screened.