茶多酚与茶黄素对前脂肪细胞3T3-L1增殖与分化的影响

Effects of tea polyphenols and theaflavins on proliferation and differentiation of 3T3-L1 preadipocytes

利用MTT法检测了经不同浓度茶多酚和茶黄素复合物处理24 h后的3T3-L1细胞的存活率,倒置显微镜观察细胞的外观形态,油红O染色法检测细胞的分化程度,并检测了分化成熟的3T3-L1细胞内的甘油三酯含量。结果表明:经茶多酚与茶黄素处理后,3T3-L1细胞的存活率明显下降,且以60μg/mL的浓度处理时,细胞存活率最低;贴壁细胞数量明显减少,贴壁细胞变大变圆;茶多酚与茶黄素明显抑制了3T3-L1前脂肪细胞的分化,分化细胞内甘油三酯的含量明显降低。表明茶多酚与茶黄素对前脂肪细胞3T3-L1的增殖和分化具有较好的抑制作用。

Cell viability was investigated by MTT assay after 3T3-L1 preadipocytes composed to tea polyphenols and theaflavins at different concentrations for 24 h,and morphological changes of adhesive 3T3-L1 preadipocytes were observed through an inverted microscope.Meantime,preadipocytes differentiation was detected by oil red O staining,and the triglyceride content of 3T3-L1 cells was also measured.The results showed that 3T3-L1 cell viability decreased significantly,especially,at 60 μg/mL concentration of tea polyphenols and theaflavins.The number of 3T3-L1 preadipocytes adhering on plate decreased and preadipocytes became round globular and bigger than the control preadipocytes.3T3-L1 preadipocytes differentiation was significantly inhibited by tea polyphenols and theaflavins,and triglyceride levels of 3T3-L1 cells was also significantly decreased.These results indicated that proliferation and differentiation of 3T3-L1 preadipocytes were effectively inhibited by tea polyphenols and theaflavins.