摘要
根据Ⅰ型鸭肝炎病毒(DHV-Ⅰ)基因组序列设计并合成引物,通过RT-PCR方法对鸭肝炎病毒SN株和弱毒疫苗株全基因分段扩增并克隆测序。比较分析SN株、弱毒疫苗株和参考毒株多聚蛋白基因和VP1基因核苷酸序列构建系统进化树。结果表明,所测定的2个毒株多聚蛋白基因核苷酸序列与DHV-Ⅰ型GFS99广东株相似性最高(99.5%)。DHV-ⅠVP1基因核苷酸序列系统进化树分为3个群:SN株在以韩国强毒株R85952为代表的强毒株群中,弱毒疫苗株在以A66为代表的弱毒株群中,另外一群是以SY1为代表的从浙江分离的强毒株。
The genome sequences of duck hepatitis virus type1(DHV-1) SN strain and attenuated vaccine strain were amplified by RT-PCR with the primers designed according to the conserved region of DHV-1 genome.The sequence of polyprotein gene and VP1 gene were analyzed by DNAStar software and the phylogenetic trees were constructed.The results showed that the two strains had closest relationship with DHV I GFS99 strain sharing 99.5% identity.Phylogenetic tree analysis of VP1 showed that all DHV-1 strains could be classified into three distinct genetic subgroups: SN strain was genetically close to R85952 virulent reference strain could be classified as subgroup;the attenuated vaccine strain was closer with low virulent A66 strain,which were classified as subgroup Ⅱ,and the SY1 virulent reference strain which isolated from Zhejiang belong to the subgroup Ⅲ.
出处
《广东农业科学》
CAS
CSCD
北大核心
2011年第12期140-141,159,共3页
Guangdong Agricultural Sciences
基金
国家自然科学基金(30170699
30871878)