枸杞属两个种的RAPD遗传多态性分析

The genetic polymorphic research between two species Lycium barbarum using RAPD marker

采用优化的CTAB法提取"宁杞1号"和"黑果枸杞"的叶片基因组DNA,利用紫外分光光度计、琼脂糖凝胶电泳检测所得DNA的浓度和纯度,并进行PCR扩增。从101个引物中筛选出12个10 bp的随机引物对这两种枸杞的DNA样品进行RAPD扩增,共得到111条谱带,61条表现出多态性,多态性比率达54.95%。结果表明,"宁杞1号"与"黑果枸杞"的相似系数S=0.618,遗传距离D=0.382。

The experiment optimized CTAB method to extract＂Lycium barbarum L.NO.1＂and ＂Lycium ruthenicum Murr.＂laminas genomic DNA.The concentration and purity of DNA were detected by UV spectrophotometer,agarose gel electrophoresis,and conducted a PCR amplification.12（10 bp）random primers were From 101 oligonucleotide primers on these two Lycium L.DNA samples amplified analysis,and 111 RAPD bands was detected,61 among which were polymorphic,the percent of polymorphic band was 54.95%.The results showed that the similarity factor of＂Lycium barbarum L.NO.1＂and＂Lycium ruthenicum Murr.＂was S=0.618,the heredity was away from D=0.382.Therefore,the genetic relationship of ＂Lycium barbarum L.NO.1＂and＂Lycium ruthenicum Murr.＂ was far.