猪抗酶基因1(AZ1)5’调控区单核甘酸多态性与屠宰性状的相关性分析

Association analysis between variants in 5' flanking region of porcine antizyme 1 gene and slaughter traits

通过PCR-RFLP方法检测了长白与蓝塘猪F2代群体抗酶基因1(AZ1)5’调控区-792^-433单核苷酸多态性,共获得3个SNP:-731C>T,-584A>C和-476G>A。-713位点处T为优势等位基因,频率为0.777,优势基因型TT频率为0.598;-584位点处A为优势等位基因,频率为0.769,优势基因型AA频率为0.599;-476位点处G为优势等位基因,频率为0.872,优势等位基因型GG频率为0.776。单倍型分析结果表明TAG(-713、-584和-476)的频率较高,为74.2%,表明这3处突变T、A和G碱基的连锁程度很高。3个SNP多态性与猪屠宰性状相关性分析结果表明,-713位点处,后躯瘦肉重和眼肌面积均是TT型大于TC型,差异极显著;而对于中躯皮脂重,TC型显著高于TT型,大理石评分则是TT型最低。-584位点处,CC基因型大理石纹型评分最高,与AA型差异显著,与CA型差异不显著。-476位点处,AA型的中躯皮脂重最小,与AG型差异显著。

With PCR-RFLP method,the 5＇ flanking region of antizyme1 gene from-792 to-433 were investigated for single nucleotide polymorphism in Landrace × Lantang F2 population.Three SNPs（-731CT,-584AC,-476GA） were identified.At-731,the ＂T＂ was preponderant allele（0.777） and ＂TT＂ was preponderant genotype（0.598）.The frequencies of allele ＂A＂ and genotype ＂AA＂ at-584 were 0.769 and 0.599,respectively.The preponderant allele and genotype at-478 were ＂G＂（0.872） and ＂GG＂（0.776）.The result of haplotype analysis showed that one major block of strong LD between the SNPs at-713,-584 and-476.Three putative haplotypes were detected,and the major haplotypes of TAG exhibited a high frequency of 74.2%.The association between genotype at-713 and slaughter traits showed that there was a clear trend toward genotype TT having greater back lean weight and genotype TC having less one.The middle skin fat of genotype TC at-713 was greater than that of TT,while the TT genotype had the lowest score of marble.At-584,the score of marble of CC was higher than the other genotypes.At-476,the middle skin fat of genotype AA was least and there had a significant difference between genotype AG and AA.