N亚型流感病毒分型基因芯片检测方法的建立

Establishment of the Detected Method of Grouping the Gene Chip of N Subtypical Influenza Virus

利用生物信息学软件对流感病毒9种N亚型基因序列进行分析筛选,设计用于9种N亚型流感病毒基因芯片分型的特异性探针,制备了针对N1~N9亚型流感病毒的鉴定基因芯片,对9种N亚型流感病毒基因芯片分型检测方法进行初步研究。结果表明：所建立的N亚型流感病毒分型芯片检测方法可以检测A型流感病毒,可对N1~N9亚型进行特异性区分,待检样品的拷贝数需大于1×102个/μL,并且重复性好,稳定性高。

Utilizing biographical information,the sequence of 9 kinds of N subtypes about influenza virus was analyzed and sifted.The designing is used to identify the specific probing pin of the 9 kinds of N subtypes about influenza virus.Aimed at identifying the N1—N9 subtypical influenza virus,it is prepared the gene chip.It provides the efficient checking technology in the research of the tentative program on the detected method of the grouping the gene chip of N subtypical influenza virus.The results indicate that the method established in this study about N subtypes about influenza virus can detect a influenza virus,and can be used for distiguishing N1—N9 subtypes,influenza virus sample copy number should be more than 1×102 ind/μL,and it is of repeatability and high stability.Kew words： influenza virus;N subtype;gene chip