不同条件对迎春花粉生活力的影响

Effects of different conditions on Jasminum nudiflorum pollen viability

以迎春花粉为试材,利用离体培养法、碘-碘化钾染色法和氯化三苯基四氮唑染色法对花粉生活力进行测定,研究不同贮藏条件对花粉萌发的影响。结果表明:培养基中添加不同浓度的蔗糖和硼酸对花粉萌发有很大影响,花粉萌发的最适培养基配方为蔗糖浓度25%、硼酸浓度0.005%,此时萌发率达92.04%。花粉生活力随贮藏时间的延长而下降,不同的贮藏条件对其有显著的影响,最佳贮藏条件为-80℃下干燥,耐贮藏时间超过26 d。

Take the fresh pollen of Jasminum nudiflorum Lindl as experiment material,germination in vitro,I2-K1 staining,TTC staining methods were used to determine the fresh pollen ability.Effects of different storage on the pollen germination were studied also.The results showed that culture media with different concentration of sucrose and boric had good effects on the pollen germination.The pollen germinating percentage reached the highest after incubated on the media supplemented with 25% sucrose and 0.005% Boric acid,the germination of pollen reached 91.04%.Vitality of Pollen were gradually descend as last of store time,it was the best methods to keep vitality of pollen at 80℃,ability of storage lasted for 26 days.