食管癌患者血清中巨噬细胞抑制因子1水平检测的意义

The significance of detecting serum MIC-1 in esophageal cancer

目的研究巨噬细胞抑制因子-1(MIC-1)在食管癌患者血清中的浓度及其临床应用价值。方法采用双抗体夹心ELISA法检测141例食管癌和3例食管良性疾病患者及200例正常对照人群血清MIC-1浓度,采用电化学发光免疫分析仪检测上述血清标本中CA19-9、CEA浓度。结果食管癌组MIC-1浓度显著高于正常对照组(P<0.001)和良性疾病组;根据ROC曲线,当550 pg/ml作为MIC-1诊断食管癌的临界值时,特异性和敏感性达到最佳,分别为76.7%和82.3%,诊断价值优于CA19-9和CEA;MIC-1浓度随着患者TNM分期的增加而增加,但转移患者(Ⅳ期)MIC-1浓度下降。结论本研究首次确认MIC-1可能成为食管癌新的血清标志物,在食管癌的诊断中,特别是在早期食管癌的诊断方面MIC-1显示出良好的应用前景。

Objective To analyze the serum concentration and its application value of macrophages inhibitory cyto- kine-1 （MIC-1） in patients with esophageal cancer. Methods Double antibody sandwich ELISA method was used to detect the serum MIC-1 level of 141 patients with esophageal cancer, 3 patients with benign esophageal diseases and 200 healthy persons （as normal control）. ECL was used to detect the serum CA19-9 and CEA concentration of these sam- pies. Results MIC-1 concentration in esophageal cancer patients was significantly higher than that in normal control （P 〈0. 001 ） and benign disease group. According to ROC curve, when 550 pg/ml was set as the cut-off value for the diagnosis of esophageal cancer, the specificity and sensitivity of MIC-1 achieved 76. 7% and 82. 3% , which were supe- rior to CEA and CA19-9. MIC-1 concentration mcreased with TNM staging, but the patients with metastatic carcinoma （stage IV） had a lower serum MIC-1 level. Conclusion MIC-1 may become a novel serum marker of esophageal cancer and it has certain application value in the diagnosis of esophageal cancer, especially in the early stage of esophageal cancer,