摘要
细菌来源的群体感应信号分子能诱导与调控植物的抗病性与生长发育,本文用细菌群体感应信号分子N-3-oxo-hexanoyl-homoserine-lactone(OHHL)对拟南芥进行不同时间的处理,提取蛋白进行双向电泳分析,用蛋白组学的方法解析拟南芥响应细菌信号分子的机制。双向电泳与质谱分析共鉴定出47个点,这些蛋白中随处理时间的增加表达量上调的蛋白点数目增加,并且与植物抗氧化、物质代谢和细胞信号转导密切相关。因此通过蛋白组学分析结果可以更好的解释植物与细菌的相互作用机制,进一步利用其之间的联系来促进植物更好的生长发育。
The populational signals derived from bacteria can induce plant resistance to pathogen or regulate plant development. Here we used 2-D proteomics method to investigate Arabidopsis thaliana seedlings responding to OHHL, one of bacterial popular signals. After 2-D proteomics and assay, we obtained 47 differential protein spots, most of their expression show increase with the extension of treatment time ; these proteins are also involved in plant antioxidant activity, material metabolism and signal transaction. Therefore, we believe our results will show more in- sight into understanding the interactions mechanism of plant and microbe, and further well regulate plant themselves growth and development.
出处
《植物分类与资源学报》
CAS
CSCD
北大核心
2011年第4期389-395,共7页
Plant Diversity
基金
国家自然科学基金面上项目(30871704
30670030
30971452)
中国科学院百人计划项目
