摘要
为优化大豆体细胞胚再生植株的条件,提高大豆遗传转化的效率,本研究选用大豆品种合丰25为试验材料,针对影响体细胞胚诱导的3个关键因素pH值、2,4-D浓度及光照条件进行体细胞胚发生频率的研究。结果表明:pH值、2,4-D浓度、光照条件这3种因素的不同处理对体细胞胚的诱导影响显著,其幼胚的发生频率最高为70.70%、最低为3.70%。当pH值为7.0、2,4-D浓度为20mg/L,黑暗培养最有利于合丰25体细胞胚的诱导,其胚的发生频率为70.70%,明显高于其他处理。
To optimize regeneration conditions of soybean somatic embryo and to improve efficiency of soybean genetic transformation,the pH value,2,4-D concentration and illumination condition were studied using "Hefeng 25" as the test material.The results showed that the different treatments of the three factors had a significant influence on the development of somatic embryo.The highest efficiency was 70.70%,while the lowest efficiency was 3.70%.When the pH value was 7.0,2,4-D concentration was 20mg/L and the materials were cultured in dark,the efficiency of soybean somatic embryo could reach 70.70%,much higher than that of other treatments.
出处
《作物杂志》
CAS
CSCD
北大核心
2011年第4期23-28,共6页
Crops
基金
教育部新世纪人才培养计划项目(NCET-09-164)
国家自然科学基金(30972889
31071439)
黑龙江省自然科学基金(C200814)
黑龙江省新世纪优秀人才培养计划项目(NCET-06-007)
中国博士后基金(20060400835)
黑龙江省博士后基金(LRB06-010)
博士后落户黑龙江科研启动基金资助项目(LBH-Q05032)
关键词
合丰25
体细胞胚
大豆再生
优化
Hefeng 25
Somatic embryos of soybean
Soybean regeneration
Optimization