厌氧活性污泥发酵制氢系统中的同型产乙酸菌及耗氢作用

Homoacetogens and Hydrogen Consumption in Anaerobic Activated Sludge Bio-hydrogen Production System

在对连续流搅拌槽式反应器(CSTR)发酵产氢系统中的活性污泥进行分子生物学分析,判断系统中有同型产乙酸菌存在的基础上,通过活性污泥的间歇培养试验,探讨了同型产乙酸作用对活性污泥发酵系统产氢效能的影响。结果表明,CSTR发酵产氢系统的活性污泥中,一种隶属真杆菌属(Eubacterium)的同型产乙酸菌在活性污泥微生物群落中达到了优势程度;以葡萄糖为底物时,同型产乙酸菌的耗氢代谢,可使厌氧活性污泥对葡萄糖的氢气转化率及产氢率分别降低31%和34%,耗氢速率可达0.33mmol/(g·d)。

The impact of homoacetogenesis on the fermentative hydrogen production of the anaerobic activated sludge in a Continuous Stirred-Tank Reactor （CSTR） is investigated by batch cultures. The predominant species in the sludge are identified with the technique of Poly-merase Chain Reaction - Denaturing Gradient Gel Electrophoresis （PCR-DGGE）, and it is indicated that the homoacetogen Eubacterium exists in the activated sludge in a remarkable dominance. The hydrogen conversion rate of glucose and the specific hydrogen production rate by the activated sludge in terms of Mixed Liquor Volatile Suspended Solids （MLVSS） are reduced by 31% and 34%, respectively, due to the etabolism of the homoacetogens in the activated sludge. The hydrogen consumption rate of the homoacetogens in the activated sludge reaches 0.33 mmol/（g.d）.