小牛血红细胞提取物眼用凝胶治疗兔角膜损伤的实验研究

Experiment study of bred cell calf blood extract eye gels in the treatment of rabbit corneal ulcer rehabilitation

目的研究小牛血红细胞提取物眼用凝胶对兔角膜损伤后创伤修复以及对角膜新生血管生成的影响。方法制作角膜板层切除模型,在兔右眼角膜中央,用直径8.5 mm环钻制作成直径8.5 mm、深度100μm的无菌性角膜损伤。新西兰大白兔40只随机分为4组(每组10只)。分别于角膜板层切除术后第1天起,各组分别用0.9%生理盐水(模型组)、重组牛碱性成纤维细胞生长因子眼用凝胶(阳性对照Ⅰ组)、50%小牛血去蛋白提取物眼用凝胶(阳性对照Ⅱ组)及20%小牛血红细胞提取物眼用凝胶(试验组)点眼每日4次。分别于治疗后7,14,21和28天,进行角膜荧光素钠染色,并观察各组角膜上皮修复情况和角膜新生血管生长情况。结果在角膜板层切除后,观察期末,20%小牛血红细胞提取物眼用凝胶组,对角膜上皮修复速度,与其他阳性对照组比较无明显差异(P>0.05);角膜新生血管生长,较其他阳性对照组明显减少(P<0.05)。结论小牛血红细胞提取物眼用凝胶明显能促进兔角膜损伤的组织修复,减少角膜新生血管形成。

Objective To observe the effect of bred cell calf blood extract eye gels in the treatment of rehabilitation of rabbit corneal unlcer and neovascularzation of the cornea.Methods One hundred μm depth sterile corneal ulcer was made in the center of right eye of all rabbit with 8.5 mm diameter trephine.Forty rabbits were randomly devided into four groups（10 rabbits for each group）,that was 0.9% normal salin group（model group）,bFGF eye gel group（controlⅠgroup）,protein-free calf blood extract eye gel group（control Ⅱgroup） and bred cell calf blood extract eye gels group（test group）.All medication was used four times per day and continued for 28 days.Epithelial defect restoration and cornea neovascularzation,etc.were observed and documented on 7,14,21,28 d after treatment.Results At the end of the observation-period,there was no significant differencen between test group and other positive control groups（P0.05）,but test group shows less neovascularzation than other positive control groups（P0.05）.Conclusion Bred cell calf blood extract eye gels has marked facilitation effect in the healing course of rabbits＇s corneal ulcer without much neovascularzation.