四逆散冻干粉中的柴胡皂苷C对小鼠睡眠影响的实验研究

Experimental study on the effects of Saikosaponin C in freeze-dry powder of Si-Ni-San on sleep time in mice

目的研究四逆散冻干粉中主要成分柴胡皂苷C对小鼠睡眠的影响。方法将小鼠随机分成3组:空白对照组、模型组和给药组,四逆散冻干粉中有效成分柴胡皂苷C 12 g.kg-1经灌胃给予给药组;空白对照组、模型组均给予同体积的蒸馏水。于末次给药前8 h禁食,给药60 min后,腹腔注射50 mg.kg-1戊巴比妥钠,观察并记录翻正反射消失至恢复时间,即睡眠时间。结果柴胡皂苷C可明显延长戊巴比妥钠所致小鼠睡眠时间,与空白组比较具有极显著性差异(P<0.01)。结论四逆散冻干粉中有效成分柴胡皂苷C可延长阈剂量戊巴比妥钠所致小鼠睡眠时间,效果明显。

Objective To study on the effects of Saikosaponin C in freeze-dry powder of Si-Ni-San on sleep time in mice.Methods Effects of Saikosaponin C in freeze-dry powder of Si-Ni-San on the prolonged the sleep time in mice induced by sodium pentobarbital was observed.Results There was significantly prolonged the sleep time of Saikosaponin C in mice.Conclusion Saikosaponin C in freeze-dry powder of Si-Ni-San had hypnotic efficacy on the sleep time in mice induced by pentobarbital sodium.