摘要
目的:研究龙葵药材中澳洲茄边碱的最佳提取工艺。方法:以澳洲茄边碱提取率为指标,采用L9(34)正交试验,考察乙醇体积分数、料液比、提取时间及提取次数对提取率的影响,优选出最佳的提取工艺。用高效液相法测定澳洲茄边碱的含量。结果:优选出的澳洲茄边碱最佳提取工艺为用80%乙醇20倍量回流提取2次、每次4 h。结论:提取工艺合理可行,适用于龙葵中澳洲茄边碱的提取。
Objective：To study the optimum extracting process of solamargine from Solanum nigrum Method：The optimum extraction process was investigated by L9（34） orthogonal design with the yield of solamargine as index,and four factors were chosen,including ethanol concentration,solid-liquid ratio,extraction times and extraction time.HPLC was used to determine the content of solamargine.Result：The optimum extraction conditions was to reflux the herbs for two times,four hours each time with 20 times amount of 80% ethanol.Conclusion：The selected technology is reasonable and feasible,and was suitable for extraction of solamargine.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第17期5-7,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技重大专项重大新药创制项目(2009ZX09103-389)
关键词
龙葵
澳洲茄边碱
提取工艺
正交试验
Solanum nigrum
solamargine
extraction process
orthogonal design
