摘要
目的:建立栀子金花丸中芒果苷的含量测定方法。方法:采用反相高效液相色谱法,Diamonsil-C18柱,乙腈-0.2%磷酸水溶液(13∶87)为流动相,流速1.0 mL.min-1,检测波长258 nm,柱温35℃。结果:芒果苷质量浓度在5.1～30.6 mg.L-1具有良好的线性关系(r=0.999 6),芒果苷平均回收率为99.0%,方法精密度RSD 1.7%(n=6)。结论:该法可用于栀子金花丸中芒果苷的含量测定。
Objective：To establish the method for determination of mangiferin in Zhizi Jinhua Wan.Method： An RP-HPLC method was established.The chromatographic column was Diamonsil-C18.The mobile phase was a mixture of acetonitrile-0.2% phosphoric acid（13∶87）.The flow rate was 1.0 mL·min-1 and the detection wavelength was 258 nm.Result： The linear range of mangiferin was 5.1-30.6 mg·L-1（r=0.999 6）.The average recovery for mangiferin was 99.0%,precision of the method was 1.7%（RSD,n=6）.Conclusion： The method can be used for quantitative determination of the preparation.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第17期65-67,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广东省自然科学基金项目(5002841)
