HPLC测定柴芍丸中芍药苷、栀子苷及丹皮酚的含量

Determination of Paeoniflorin,Geniposide and Paeonol in Chaishao Pills by HPLC

目的:建立以高效液相色谱法同时测定柴芍丸中芍药苷、栀子苷及丹皮酚含量的方法。方法:色谱柱为InertsilODS-SP(4.6 mm×150 mm,5μm),流动相乙腈-水梯度洗脱,检测波长230 nm,流速1.0 mL.min-1。结果:芍药苷在0.494 4～12.36μg(r=1)线性关系良好,平均回收率为98.77%,RSD 2.1%;栀子苷在0.492 2～12.305μg(r=0.999 9)线性关系良好,平均回收率101.64%,RSD 1.9%;丹皮酚在0.260 2～6.505μg(,r=0.999 9)线性关系良好,平均回收率100.92%,RSD1.7%。结论:方法简便、快速、重复性好,可用于柴芍丸的质量控制。

Objective：To establish an HPLC method for the simultaneous determination of paeoniflorin,geniposide and paeonol in Chaishao Pills.Method： Paeoniflorin,geniposide and paeonol were separated on Inertsil ODS-SP（4.6 mm×150 mm,5 μm） column and detected at 230 nm.The mobile phase was acetonitrile-water with gradient elution.The flow rate was 1.0 mL·min-1.Result： Paeoniflorin,geniposide and paeonol were linear within the range of 0.494 4-12.36 μg（r=1）,0.492 2-12.305 μg（r=0.999 9） and 0.260 2-6.505 μg,（r=0.999 9） respectively.The average recovery was 98.77%（2.1%）,101.64%（1.9%） and 100.92%（1.7%）.Conclusion： The method is simple,reproducible,and it can be used for the quality control of Chaishao pills.