蔓荆子种子生活力的测定及其与发芽率的相关性研究

Measurement of Seed Viability and Correlation with Germination Rate for Vitex trifolia L.var.simplicifolia Cham.

目的:筛选测定蔓荆子种子生活力的最佳方法,明确生活力与发芽率之间的相关性。方法:以5个不同产地的蔓荆子作为实验材料,分别用四氮唑染色法、红墨水染色法、BTB指示剂法测定种子的生活力。测定不同培养床(滤纸、海绵、沙子、蛭石)和不同温度(15,20,25,30,30/20,25/15℃)对发芽率的影响,优化种子发芽条件。结果:四氮唑染色法测定种子的生活力是3种测定方法中最准确的,在蛭石培养床、30℃恒温培养条件下,蔓荆子的发芽率最高。四氮唑染色法测定的蔓荆子生活力与发芽力比值为0.992,存在显著的正相关性,进一步求得蔓荆子四氮唑测定生活力X与发芽率Y的回归方程为Y=0.967X+6.038。结论:四氮唑染色法是蔓荆子种子生活力测定的最佳方法,生活力与发芽率呈显著的正相关。

Objective：To screen the optimized seed viability test methods for Vitex trifolia L.var.simplicifolia Cham,and then measure the correlation between the germination rate and seed viability.Method：TTC（2,3,5-triphynel tetrazolilum chroride） staining,red ink staining and BTB staining were used respectively to evaluate seed viability of V.trifolia L.var.simplicifolia from five habitats.Conditions for seed germination contained four kinds of culture beds（filter paper,sponge,sand and vermiculite） and six temperature（15,20,25,30,30 ℃ then shift to 20 ℃,25 ℃ then shift to 15 ℃）.Result：Compared with the other two methods,TTC staining was the best one for the seed viability test.The seeds can reach to the highest germination rate on vermiculite culture bed in 30 ℃.There was a significant relationship between germination rate and seed viability tested by TTC staining,and the correlation coefficient can reached to 0.992.The regression equation was proved to be Y=0.967X＋6.038（X：seeds viability;Y：germination rate）.Conclusion：TTC staining was the optimal method to determine seed viability of V.trifolia L.var.simplicifolia.seed viability tested by this method was significant related with germination rate.