壮骨止痛方无水乙醇提取A部位对去势雌鼠骨质疏松的疗效及机制研究

Efficacy of Ethanol Extracted Part A of Zhuanggu Zhitong Prescription for Osteoporosis in Mice

目的:观察壮骨止痛方无水乙醇提取A部位对去势雌鼠骨质松模型的疗效,并探讨其可能的作用机制。方法:选取250 g左右SD雌性大鼠75只,按体重随机分为模型组、假手术组、正常组、壮骨止痛方无水乙醇提取A部位组(0.12 g.kg-1.d-1)、壮骨止痛方组(13.2 g.kg-1.d-1),每组15只。去卵巢术后5 d开始给药,连续13周。取血和骨标本从骨密度、骨生物力学、病理形态学等几个方面研究其药效,从血清雌激素(E2)、骨钙素(BGP)、甲状旁腺素(PTH)、降钙素(CT)方面研究其作用机制。结果:壮骨止痛方无水乙醇提取A部位能明显增加去卵巢模型鼠股骨、腰椎骨的骨密度(P<0.05);显著提高股骨头平均骨小梁密度(P<0.01);显著增强模型大鼠胫骨的最大应力和最大抗弯强度(P<0.01);与去势模型组比较壮骨止痛方无水乙醇提取A部位明显增加子宫系数(P<0.05),能显著降低去势模型鼠血清CT(P<0.01)。结论:壮骨止痛方无水乙醇提取A部位能增加模型鼠骨密度及骨最大应力和最大抗弯强度,逆转骨质疏松症的病理形态学改变。其作用机制之一可能是发挥药物自身的雌激素样作用,改善激素代谢紊乱,直接或间接调节了机体的骨代谢和细胞因子,降低了骨转换,促进骨形成,抑制骨吸收。

Objective：To observe the effects of alcohol extracted part A of Zhuanggu Zhitong prescription for treatment of osteoporosis,and to explore the possible mechanism.Method： Seventy five female SD rats weighing about 250 g were randomly divided into control group,model group（induced by ovariectomization）,sham operation group,ethanol extracted partA of Zhuanggu Zhitong prescription group（0.12 g·kg-1·d-1） and Zhuanggu Zhitong prescription group（13.2 g·kg-1·d-1）.Five days after ovariectomization,different treatment were lasted for 13 weeks.Blood and bone samples of the rats were used to determine the bone mineral density,bone biomechanics,path-ology and other aspects to evaluate the efficacy.Serum（estrone）（E2）,bone gla protein（BGP）,parathyroid gland（PTH）,calcitonin（CT） were investigated for exploring the related mechanism.Result： Ethanol extracted part A of Zhuanggu Zhitong prescription showed significantly increase in femoral and lumbar bone mineral density（P0.05）;could significantly enhance the maximum stress and tibia maximum flexural strength（P0.01）;Ethanol extracted part A had an increasing trend of serum E2 and uterine wet weight.Ethanol extracted part A could significantly increase the average trabecular bone-density of the femoral head（P0.01）.Ethanol extracted part A could significantly decreasethe serum CT（P0.01）.Conclusion： The ethanol extracted part A can increase the bone density,the maximum stressand the maximum bending strength.Part A may reverse osteoporosis with improved pathological evidences.The mechanism may be related to estrogen-like action of the drug with improved hormone metabolism,regulated bone metabolism and cytokines,reduced bone turnover,bone formation and inhibited bone resorption.