养心调肝益肾煎剂对去势雄性大鼠的壮阳作用

Strengthening Yang Action of Yangxin Tiaogan Yishen Decoction in Catrated Male Rats

目的:研究养心调肝益肾煎剂壮阳作用及其可能机制。方法:手术去势造模,造模后第8天对不同实验组分别ig生理盐水(NS)、养心调肝益肾煎剂高、低剂量(50,25 g.kg-1)、男宝混悬液(0.56 g.kg-1),连续用药21 d后测量去势雄鼠附性器官包皮腺、精液囊-前列腺、提肛肌的质量,计算其脏器系数,测量血清睾酮(T)、皮质醇(F)及阴茎海绵体内皮细胞一氧化氮(NO)的含量。结果:养心调肝益肾煎剂组与模型组比较,有明显增加去势雄鼠包皮腺、精液囊-前列腺、提肛肌脏器系数及血清T、F及阴茎NO含量的作用(P<0.05),尤以养心调肝益肾煎剂高剂量组更为明显(P<0.05)。结论:养心调肝益肾煎剂可调节下丘脑-垂体-性腺轴及肾上腺皮质系统功能,具有壮阳作用。

Objective：To investigate strengthening yang action of Yangxin Tiaogan Yishen decoction and its possible mechanism.Method：On the 8th days after making castrated model rats by operation,we respectively fed NS,Yangxin Tiaogan Yishen decoction（50,25 g·kg-1）,Nanbao mixed liquid（0.56 g·kg-1） into the stomaches of the different groups of male rats for 21 days.Afterwards,the weight of prepputial gland,sperm bladder-prostate,anus levator muscle,content of testosterone（T） and cortisol（F） in blood serum and nitric oxide（NO） content of endothelium cell of penial corpus cavernosum were observed.Result：The indexes of prepputial gland,sperm bladder-prostate,anus levator muscle,and content of T,F and NO were increased,these items of Yangxin Tiaogan Yishen decoction group were higher than these of the model group（P0.05）.The deta in Yangxin Tiaogan Yishen decoction with high dose group were especially remarkable（P0.05）.Conclusion：Yangxin Tiaogan Yishen decoction can adjust lower thalamus-hypophysis-sexual gland axis and adrenal function,and it can strengthen yang action.