青蒿素及其类似物提取及分析方法研究进展

Progress of Extraction and Analytical Methods of Artemisinin and Its Analogues

目的:介绍青蒿素及其类似物的提取及分析检测方法,为青蒿的深度研发提供参考。方法:查阅近年国内外有关青蒿素及其类似物提取及分析方法研究方面的文献33篇,并对其进行归纳、分析和总结。结果:目前关于青蒿素及其类似物类化合物的提取方法主要包括有机溶剂回流提取法、超声波辅助提取法、微波辅助提取法、超临界二氧化碳辅助提取法;含量分析方法主要有质量法,紫外分光光度法,近红外光谱法,薄层色谱法,气相色谱-电化学检测法(质谱法、氢火焰离子化法),液相色谱-紫外分光光度法(视差检测法、蒸发光散射检测法、质谱法、电化学检测法及毛细管电泳法),核磁共振波谱法、伏安法及生物学方法。结论:各种提取及分析方法均有其优缺点,需根据研究的目的、要求和实验条件灵活运用。

Objective：To review the extraction and analytical methods of artemisinin and its analogues,it given the reference for the research and development of Artemisia annua.Method： Literatures on extraction and analytical methods of artemisinin and its analogues in recent years were summarized and analyzed.Result and Conclusion：Currently,the extraction methods about artemisinin and its analogues include organic solvent extraction,ultrasonic extraction,microwave assisted extraction,supercritical carbon dioxide assisted extraction,the analytical methods as to these compounds were GC-ECD（MS,FID）,HPLC-UV（MS,RID,ELSD,EC,CE）,1H-NMR,voltammetry and biological methods.Each extraction and analytical method of artemisinin and its analogues have it＇s advantages and disadvantages,to be flexible use the methods depend on the purpose,requirements and actual conditions.